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If you are actually talking about bio diesel and not some blend concoction I would say yes. I have run home brewed B100 in my 7.3 for thousands of miles.
You may have to change the fuel filter a time or two at first depending on how clean or dirty your fuel system is.
You can cut it with dino for cold temps. 70/30 should get you well below freezing, the oil stock and quality can have some effect on that.
You can heat the tank as well but I never have, so I can't speak about that.
To my knowledge there is nothing different about a 6.0 that would keep you from using Bio unless it has a DPF or Cat Converter. If it has either I think they need to be removed first.
Big Red = 2001 F250 4X4 Crew Cab Short Bed 108K mi.
TS 6 Position Chip, Tony Wildman Tunes , MBRP Stainless 4 inch exhaust
K&N intake, Zoodad mod, HX crossover. Optix boost, pyro and trans temp gauges.
Rebuilt trans with Tugger shift kit and 40,000 gvw TruCool cooler.
Running B100 home brew bio.